Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A Rab1 interactome illuminates a dual role in autophagy and membrane trafficking.

van Vliet AR, Gillingham AK, Morgan TE, Ohashi Y, Smith TS, Abid Ali F, Munro S

The small GTPase Rab1 is found in all eukaryotes and acts in both ER-to-Golgi transport and autophagy. Several Rab1 effectors and regulators have been identified, but the mechanisms by which Rab1 orchestrates these distinct processes remain incompletely understood. We apply MitoID, a proximity biotinylation approach, to expand the interactome of human Rab1A and Rab1B. We identify new interactors among known ... [more]

J Cell Biol Mar. 02, 2026; 225(3); [Pubmed: 41493270]

Throughput

  • High Throughput

Additional Notes

  • GTP-bound form (Q67L mutant)
  • MitoID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAB1B COG2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3365672

Curated By

  • BioGRID