BAIT

DCAF4

WDR21, WDR21A
DDB1 and CUL4 associated factor 4
UPS Project
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

CCT5

CCT-epsilon, CCTE, HEL-S-69, TCP-1-epsilon
chaperonin containing TCP1, subunit 5 (epsilon)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation.

Yamanaka S, Nagaoka K, Shoya Y, Nishino K, Mikura Y, Tanaka K, Konishi K, Hasegawa Y, Hijikata A, Kosako H, Sawasaki T

The DDB1- and CUL4-associated factor (DCAF) family functions as substrate receptors within Cullin4-really interesting new gene (RING) ubiquitin ligases (CRL4s), facilitating proteasomal degradation of targeted substrates. Although CRL4-based targeted protein degradation (TPD) has emerged as a promising strategy to modulate undruggable proteins, the complex formation, substrates, and functional properties of many DCAFs remain poorly defined. In this study, using proximity ... [more]

Mol Cell Apr. 02, 2026; 86(7);1397-1416.e11 [Pubmed: 41932313]

Throughput

  • High Throughput

Additional Notes

  • Air-ID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DCAF4 CCT5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.67BioGRID
3244814
DCAF4 CCT5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9727BioGRID
3073550
DCAF4 CCT5
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3568386

Curated By

  • BioGRID