Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation.

Yamanaka S, Nagaoka K, Shoya Y, Nishino K, Mikura Y, Tanaka K, Konishi K, Hasegawa Y, Hijikata A, Kosako H, Sawasaki T

The DDB1- and CUL4-associated factor (DCAF) family functions as substrate receptors within Cullin4-really interesting new gene (RING) ubiquitin ligases (CRL4s), facilitating proteasomal degradation of targeted substrates. Although CRL4-based targeted protein degradation (TPD) has emerged as a promising strategy to modulate undruggable proteins, the complex formation, substrates, and functional properties of many DCAFs remain poorly defined. In this study, using proximity ... [more]

Mol Cell Apr. 02, 2026; 86(7);1397-1416.e11 [Pubmed: 41932313]

Throughput

  • High Throughput

Additional Notes

  • Air-ID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPN2 TOR1AIP2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High290BioGRID
3005265

Curated By

  • BioGRID