BAIT

CYM1

MOP112, YDR430C
Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

LAT1

ODP2, PDA2, dihydrolipoyllysine-residue acetyltransferase, L000000932, L000003242, YNL071W
Dihydrolipoamide acetyltransferase component (E2) of the PDC; the pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global mitochondrial connectivity map reveals the landscape of yeast functional assemblies and conserved protein communities.

Jessulat M, Phanse S, Aoki H, Broderick K, Zhang Q, Castro IG, Novales NA, Hossain SA, Saccon T, Moutaoufik MT, Joseph TP, Amin S, Hoell L, Minic Z, Bykov Y, Preminger N, Crespo SG, Snider J, Golshani A, Stagljar I, Rodriguez-Medina JR, Clarke CF, Schuldiner M, Babu M

Mitochondria are essential organelles whose functions depend on coordinated multiprotein complexes, yet their composition and organization remain incomplete. Here, we present a large-scale map of mitochondrial protein complexes by integrating affinity purification of 740 endogenously GFP-tagged mitochondrial proteins with biochemical co-fractionation of mitochondrial extracts from yeast (Saccharomyces cerevisiae) grown under respiratory conditions. Mass spectrometry identifies 13,716 high-confidence protein associations and ... [more]

Nat Commun May. 05, 2026; (); [Pubmed: 42086547]

Quantitative Score

  • 4.96 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • High throughput score cut-off value/description The PPIs are derived from two methods AP-MS and CF-MS. The LLS PPI score is derived from the LLS AP-MS and LLS CF-MS scores. The cutoff for AP-MS is 5.34 and CF-MS is 3.12. If the PPI is detected by both methods and meets the threshold in one of the methods the Average LLS score is taken as the final LLS score for the PPI.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CYM1 LAT1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
CYM1 LAT1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
LAT1 CYM1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-6.35BioGRID
580611

Curated By

  • BioGRID