BAIT
ILV3
dihydroxy-acid dehydratase ILV3, L000000859, YJR016C
Dihydroxyacid dehydratase; catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MSN1
FUP1, HRB382, MSS10, PHD2, L000001197, YOL116W
Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
GO Process (6)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to increased salt [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to methylglyoxal [IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
The genetic landscape of a cell.
A genome-scale genetic interaction map was constructed by examining 5.4 million gene-gene pairs for synthetic genetic interactions, generating quantitative genetic interaction profiles for approximately 75% of all genes in the budding yeast, Saccharomyces cerevisiae. A network based on genetic interaction profiles reveals a functional map of the cell in which genes of similar biological processes cluster together in coherent subsets, ... [more]
Science Jan. 22, 2010; 327(5964);425-31 [Pubmed: 20093466]
Quantitative Score
- -0.1757 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- A Synthetic Genetic Array (SGA) analysis was carried out to quantitatively score genetic interactions based on fitness defects that were estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an SGA score of epsilon > 0.08 for positive interactions and epsilon < -0.08 for negative interactions, and a p-value < 0.05.
- YJR016C is an essential gene and therefore a gene-dosage DAmP allele was used in the experiment. The Decreased Abundance by mRNA Perturbation (DAmP) method results in destabilizing a gene's transcripts thereby decreasing the amount of protein.
Curated By
- BioGRID