QCR6
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
QCR10
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Global mitochondrial connectivity map reveals the landscape of yeast functional assemblies and conserved protein communities.
Mitochondria are essential organelles whose functions depend on coordinated multiprotein complexes, yet their composition and organization remain incomplete. Here, we present a large-scale map of mitochondrial protein complexes by integrating affinity purification of 740 endogenously GFP-tagged mitochondrial proteins with biochemical co-fractionation of mitochondrial extracts from yeast (Saccharomyces cerevisiae) grown under respiratory conditions. Mass spectrometry identifies 13,716 high-confidence protein associations and ... [more]
Quantitative Score
- 6.11 [Confidence Score]
Throughput
- High Throughput
Additional Notes
- High throughput score cut-off value/description The PPIs are derived from two methods AP-MS and CF-MS. The LLS PPI score is derived from the LLS AP-MS and LLS CF-MS scores. The cutoff for AP-MS is 5.34 and CF-MS is 3.12. If the PPI is detected by both methods and meets the threshold in one of the methods the Average LLS score is taken as the final LLS score for the PPI.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| QCR6 QCR10 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 6 | BioGRID | 3621115 | |
| QCR6 QCR10 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1649 | BioGRID | 537881 | |
| QCR10 QCR6 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1246 | BioGRID | 538203 | |
| QCR10 QCR6 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 162413 | |
| QCR6 QCR10 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 535037 |
Curated By
- BioGRID