BAIT

TIM11

ATP21, F1F0 ATP synthase subunit e, L000004719, YDR322C-A
Subunit e of mitochondrial F1F0-ATPase; ATPase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase, which in turn determines the shape of inner membrane cristae
Saccharomyces cerevisiae (S288c)
PREY

RDL2

AIM42, FMP31, YOR286W
Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Global mitochondrial connectivity map reveals the landscape of yeast functional assemblies and conserved protein communities.

Jessulat M, Phanse S, Aoki H, Broderick K, Zhang Q, Castro IG, Novales NA, Hossain SA, Saccon T, Moutaoufik MT, Joseph TP, Amin S, Hoell L, Minic Z, Bykov Y, Preminger N, Crespo SG, Snider J, Golshani A, Stagljar I, Rodriguez-Medina JR, Clarke CF, Schuldiner M, Babu M

Mitochondria are essential organelles whose functions depend on coordinated multiprotein complexes, yet their composition and organization remain incomplete. Here, we present a large-scale map of mitochondrial protein complexes by integrating affinity purification of 740 endogenously GFP-tagged mitochondrial proteins with biochemical co-fractionation of mitochondrial extracts from yeast (Saccharomyces cerevisiae) grown under respiratory conditions. Mass spectrometry identifies 13,716 high-confidence protein associations and ... [more]

Nat Commun May. 05, 2026; (); [Pubmed: 42086547]

Quantitative Score

  • 5.66 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • High throughput score cut-off value/description The PPIs are derived from two methods AP-MS and CF-MS. The LLS PPI score is derived from the LLS AP-MS and LLS CF-MS scores. The cutoff for AP-MS is 5.34 and CF-MS is 3.12. If the PPI is detected by both methods and meets the threshold in one of the methods the Average LLS score is taken as the final LLS score for the PPI.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TIM11 RDL2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1311BioGRID
369614

Curated By

  • BioGRID