Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The compartment-specific interactome of VDAC1 reveals its emerging role beyond mitochondria in breast cancer.

Jozwiak P, Madrecki M, Oracz J, Jankowski M, Balcerzak K, Kozal KA, Zyzelewicz D, Krzeslak A

Voltage-dependent anion channel 1 (VDAC1) is a multifunctional protein that is primarily localized in the outer mitochondrial membrane, where it regulates metabolite flux and apoptosis. Aberrant expression of VDAC1 has been implicated in tumorigenesis. Moreover, recent reports suggested that VDAC1 is localized at the plasma membrane, but its role in this compartment remains unclear. This study aimed to evaluate the ... [more]

Sci Rep Apr. 30, 2026; (); [Pubmed: 42062430]

Throughput

  • High Throughput

Additional Notes

  • mitochondrial interaction partners

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SQSTM1 VDAC1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
VDAC1 SQSTM1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID