GRIP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GRIA2
Gene Ontology Biological Process
- establishment of protein localization [ISO]
- ion transmembrane transport [IBA]
- ionotropic glutamate receptor signaling pathway [IBA, ISO]
- positive regulation of synaptic transmission [ISO]
- protein tetramerization [ISO]
- receptor internalization [ISO]
- regulation of receptor recycling [ISO]
- regulation of synaptic transmission, glutamatergic [ISO]
- synaptic transmission [IDA, ISO]
- synaptic transmission, glutamatergic [IBA]
Gene Ontology Molecular Function- PDZ domain binding [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IBA, ISO]
- extracellular-glutamate-gated ion channel activity [IDA]
- identical protein binding [ISO]
- ionotropic glutamate receptor activity [IDA, ISO]
- kainate selective glutamate receptor activity [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor activity [ISO]
- PDZ domain binding [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IBA, ISO]
- extracellular-glutamate-gated ion channel activity [IDA]
- identical protein binding [ISO]
- ionotropic glutamate receptor activity [IDA, ISO]
- kainate selective glutamate receptor activity [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor activity [ISO]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [IDA, ISO]
- asymmetric synapse [ISO]
- cell surface [ISO]
- dendrite [IBA, ISO]
- dendrite cytoplasm [ISO]
- dendritic shaft [ISO]
- dendritic spine [ISO]
- endoplasmic reticulum [IDA]
- growth cone [ISO]
- integral component of plasma membrane [ISO]
- membrane [IDA]
- neuron projection [IDA]
- neuronal cell body [ISO]
- perikaryon [ISO]
- postsynaptic density [ISO]
- postsynaptic membrane [IBA, IDA]
- presynaptic membrane [ISO]
- protein complex [ISO]
- synapse [IDA, ISO]
- synaptic vesicle [IDA]
- synaptic vesicle membrane [ISO]
- terminal bouton [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Dysregulated GluA2-Y876 phosphorylation contributes to loss of synaptic upscaling in GRIP1 mutant mice with reduced sociability and increased repetitive behavior.
Loss of synaptic upscaling, a post-synaptic homeostatic plasticity, has been reported in mouse models of autism, but the underlying mechanism remains unknown. Glutamate receptor interacting protein 1 (GRIP1) binds AMPA receptor 2 (GluA2) through its PDZ domains 4-6 where gain-of-function variants were described in autism. We characterized mice carrying one variant, GRIP1-I586L (murine I507L), that shows increased binding with GluA2. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GRIA2 GRIP1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.774 | BioGRID | 2680915 |
Curated By
- BioGRID