FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Bivalent ligands targeting oxytocin receptor homodimers selectively activate distinct G protein and ?-arrestin pathways.

Busnelli M, Gori A, Chini B

G protein-coupled receptors can assemble into both homodimers and heterodimers. While heterodimerization has been extensively characterized, the functional consequences of homodimerization on G protein coupling, signaling, and trafficking remain poorly understood. Oxytocin receptors (OTRs) form homodimers, and we previously generated a series of homobivalent compounds composed of two modified oxytocin analogs (dOTK) linked by spacers of varying length. We already ... [more]

Biochem Pharmacol May. 01, 2026; 247();117814 [Pubmed: 41707738]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GNG2 GNAI2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID