Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Exploration of the proxiOME of large subunit ribosomal proteins reveals Acl1 and Bcl1 as cooperating dedicated chaperones of Rpl1.

Favre S, Pillet B, Burchert F, Siva Sankar D, Mendez-Godoy A, Kiontke S, Dengjel J, Bange G, Kressler D

In eukaryotes, most newly synthesized ribosomal proteins (r-proteins) need to rapidly and safely get into the nucleus to reach their assembly site on pre-ribosomal particles. However, only for few r-proteins tailored support mechanisms involving so-called dedicated chaperones (DCs) could so far be revealed. Here, with the primary aim of identifying novel DCs, we performed TurboID-based proximity labelling with all 46 ... [more]

Nucleic Acids Res Mar. 19, 2026; 54(6); [Pubmed: 41909949]

Throughput

  • Low Throughput

Ontology Terms

  • hela cell (BTO:0000567) [cervical adenocarcinoma cell (BTO:0003165)]
  • epithelial cell of cervix (CL:0002535)
  • uterine cervix (BTO:0001421)

Additional Notes

  • MiniTurbo-based proximity labelling in HeLa cells

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPL10A NOP2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.8672BioGRID
1175805

Curated By

  • BioGRID