BAIT

PHB2

BAP, BCAP37, Bap37, PNAS-141, REA, p22
prohibitin 2
GO Process (1)
GO Function (2)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

LRPPRC

CLONE-23970, GP130, LRP130, LSFC
leucine-rich pentatricopeptide repeat containing
Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Cross-Linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics.

Ihling CH, Piersimoni L, Kipping M, Sinz A

The combination of cross-linking/mass spectrometry (XL-MS) and ion mobility is still underexplored for conducting protein conformational and protein-protein interaction studies. We present a method for analyzing cross-linking mixtures on a timsTOF Pro mass spectrometer that allows separating ions based on their gas-phase mobilities. Cross-linking was performed with three urea-based MS-cleavable cross-linkers that deliver distinct fragmentation patterns for cross-linked species upon ... [more]

Anal Chem Aug. 24, 2021; 93(33);11442-11450 [Pubmed: 34375526]

Throughput

  • High Throughput

Ontology Terms

  • hek-293 cell (BTO:0000007) [embryonic kidney cell line (BTO:0002733)]
  • kidney epithelial cell (CL:0002518)
  • kidney (BTO:0000671)

Additional Notes

  • HEK-293 nuclear extract

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHB2 LRPPRC
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High18.85BioGRID
2999911

Curated By

  • BioGRID