An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Auxin-induced depletion of human CCR4-NOT subunits reveals opposing functions of CNOT1 and CNOT4 in mRNA metabolism.

Kulkarni S, Smith C, Akinniyi OT, Giardine BM, Keller CA, Arnaoutov A, Reese JC

CCR4-NOT regulates multiple steps in gene regulation, including transcription, mRNA decay, protein ubiquitylation, and translation. Originally discovered in yeast, this complex is highly conserved across eukaryotes, although its composition and functions differ between mammals and yeast. For example, unlike yeast Not4, human CNOT4 (E3 Ubiquitin Ligase) does not form a stable complex with CCR4-NOT, and its functions are less clear ... [more]

J Biol Chem Dec. 01, 2025; 301(12);110862 [Pubmed: 41161383]

Throughput

High Throughput

Additional Notes

Proximity label MS was carried out to identify high confidence protein interactors with enrichment of greater than or equal to 1.5 fold and p-adj < 0.05

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DDX6 CNOT4	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Youn JY (2018)	High	-	BioGRID	2813330

Curated By

BioGRID

Interaction Summary

CNOT4

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

DDX6

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA helicase activity [TAS]helicase activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]