Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Targeting PGE2 mediated senescent neuron improves tumor therapy.

Zhao J, Wu L, Cai G, Ou D, Liao K, Yang J, Zhou L, Huang R, Lin S, Huang X, Lv Q, Chen J, Cao L, Chen J, Lin Y

Recent studies have highlighted bidirectional signaling between tumors and neurons; however, the interactions between tumors and neurons in response to radio-/chemotherapy remain obscure, which hampers the tumor treatment.Glioblastoma organoids (GBOs) and primary neuron coculture, targeted metabonomics, RNA pulldown, mass spectrum, co-immunoprecipitation, RNA-sequencing, transcript/protein validations, and multi-electrode arrays were performed to analyze neuron-tumor interaction in response to therapy. In vivo validations ... [more]

Neuro Oncol Jul. 30, 2025; 27(6);1491-1506 [Pubmed: 39963753]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FASN EIF4A1
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
2862361

Curated By

  • BioGRID