An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion.

Collotta G, Gatti M, Ungureanu IM, van Ackeren V, Rannou E, Vivalda F, Gomez Vieito D, Fishwick KM, von Aesch C, Porro A, Ungerleider K, Heidari A, Guerois R, Harding RJ, Bischof S, Balmus G, Sartori AA

Human FAN1 is a structure-specific endonuclease implicated in the repair of DNA interstrand crosslinks (ICLs) and the excision of extrahelical CAG repeats-whose pathological expansion underlies Huntington's disease (HD), a progressive and currently incurable neurodegenerative disorder. However, mechanisms of post-translational regulation of FAN1 are still largely unknown. Here, we identify the ubiquitin-specific protease 7 (USP7) as an interactor of FAN1. USP7 ... [more]

Nat Commun Mar. 06, 2026; 17(1); [Pubmed: 41786746]

Throughput

High Throughput

Additional Notes

Affinity capture MS was carried out to identify high confidence protein interactors with a log2(FC) of greater than or equal to 1 and a -log10(FDR) or greater than or equal to 2

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FAN1 FANCD2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Goold R (2021)	Low	-	BioGRID	-
FANCD2 FAN1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Goold R (2021)	Low	-	BioGRID	-
FAN1 FANCD2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	MacKay C (2010)	Low	-	BioGRID	-
FANCD2 FAN1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Unno J (2014)	Low	-	BioGRID	-
FANCD2 FAN1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Chaudhury I (2014)	Low	-	BioGRID	-
FAN1 FANCD2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Kratz K (2010)	Low	-	BioGRID	-
FAN1 FANCD2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	MacKay C (2010)	Low	-	BioGRID	-
FAN1 FANCD2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Porro A (2017)	Low	-	BioGRID	-
FAN1 FANCD2	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	MacKay C (2010)	Low	-	BioGRID	519018
FANCD2 FAN1	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Kratz K (2010)	Low	-	BioGRID	-
FANCD2 FAN1	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	MacKay C (2010)	Low	-	BioGRID	519021
FANCD2 FAN1	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Smogorzewska A (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FAN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]5'-flap endonuclease activity [IDA]magnesium ion binding [TAS]protein binding [IPI]ubiquitin binding [IDA]

Gene Ontology Cellular Component

FANCD2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA polymerase binding [IPI]protein binding [IPI]