Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Comprehensive dataset of interactors for the entire PARP family using TurboID proximity labeling.

Zheng J, Deng Y, Fang C, Xiong S, Zhu X, Wu W, Chen X, Wu W, Yin D, Hu K, Yan H

A comprehensive dataset detailing protein interactors for the PARP family has been generated using TurboID proximity labeling under standardized experimental conditions. V5-TurboID fusion constructs enabled identification of 6,314 high-confidence interacting proteins through mass spectrometry, capturing transient interactions undetectable by conventional methods. Parallel GFP-PARP localization experiments validated physiological subcellular distributions. The dataset reveals both shared and unique interactors across PARP members, ... [more]

Sci Data Mar. 08, 2025; 12(1);405 [Pubmed: 40057523]

Throughput

  • High Throughput

Additional Notes

  • Turbo ID
  • log2FC greater than 1 and P less than 0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TNKS AMOT
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Low-BioGRID
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Curated By

  • BioGRID