CEBPE
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TBX2
Gene Ontology Biological Process
- aorta morphogenesis [ISS]
- atrioventricular canal development [ISS]
- cardiac muscle tissue development [ISS]
- cell aging [IDA]
- cellular senescence [IDA]
- embryonic camera-type eye morphogenesis [ISS]
- embryonic digit morphogenesis [ISS]
- endocardial cushion morphogenesis [ISS]
- muscle cell fate determination [ISS]
- negative regulation of cardiac chamber formation [ISS]
- negative regulation of heart looping [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- outflow tract morphogenesis [ISS]
- outflow tract septum morphogenesis [ISS]
- pharynx development [ISS]
- positive regulation of cardiac muscle cell proliferation [ISS]
- positive regulation of cell proliferation [IDA]
- regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [ISS]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Exploring genetic interaction manifolds constructed from rich single-cell phenotypes.
How cellular and organismal complexity emerges from combinatorial expression of genes is a central question in biology. High-content phenotyping approaches such as Perturb-seq (single-cell RNA-sequencing pooled CRISPR screens) present an opportunity for exploring such genetic interactions (GIs) at scale. Here, we present an analytical framework for interpreting high-dimensional landscapes of cell states (manifolds) constructed from transcriptional phenotypes. We applied this ... [more]
Quantitative Score
- 6.031387677 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell proliferation (GO:0008283)
Additional Notes
- CRISPR GI Activation screen
- Cell Line:K-562 (BTO:0000664/CELLOSAURUS:CVCL_0004)
- Experimental Setup: In vitro proliferation
- GIST: A-phenotypic positive genetic interaction
- Library: CRISPRa GI library
- Significance Threshold: -2.77>GI score>2.83 (Figure 1F)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CEBPE TBX2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID