An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Defining the human deubiquitinating enzyme interaction landscape.

Sowa ME, Bennett EJ, Gygi SP, Harper JW

Deubiquitinating enzymes (Dubs) function to remove covalently attached ubiquitin from proteins, thereby controlling substrate activity and/or abundance. For most Dubs, their functions, targets, and regulation are poorly understood. To systematically investigate Dub function, we initiated a global proteomic analysis of Dubs and their associated protein complexes. This was accomplished through the development of a software platform called CompPASS, which uses ... [more]

Cell Jul. 23, 2009; 138(2);389-403 [Pubmed: 19615732]

Quantitative Score

1.45 [Confidence Score]

Throughput

High Throughput

Ontology Terms

hek-293 cell (BTO:0000007) [epithelial cell (BTO:0000414)]

Additional Notes

#LPPI
Likely protein-protein interaction
exogenous expression of bait

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
USP25 NEDD8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Xu D (2017)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

USP25

Gene Ontology Biological Process

Gene Ontology Molecular Function

SUMO binding [IDA]cysteine-type endopeptidase activity [IBA]peptidase activity [TAS]protein binding [IPI]ubiquitin thiolesterase activity [IBA]ubiquitin-specific protease activity [IMP]

Gene Ontology Cellular Component

NEDD8