BAIT

DMWD

D19S593E, DMR-N9, DMRN9, gene59
dystrophia myotonica, WD repeat containing
Autism spectrum disorder (ASD) Project
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

S100A8

60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8
S100 calcium binding protein A8
Autophagy Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Defining the human deubiquitinating enzyme interaction landscape.

Sowa ME, Bennett EJ, Gygi SP, Harper JW

Deubiquitinating enzymes (Dubs) function to remove covalently attached ubiquitin from proteins, thereby controlling substrate activity and/or abundance. For most Dubs, their functions, targets, and regulation are poorly understood. To systematically investigate Dub function, we initiated a global proteomic analysis of Dubs and their associated protein complexes. This was accomplished through the development of a software platform called CompPASS, which uses ... [more]

Cell Jul. 23, 2009; 138(2);389-403 [Pubmed: 19615732]

Quantitative Score

  • 5.77 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • hek-293 cell (BTO:0000007) [epithelial cell (BTO:0000414)]

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID