An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Defining the human deubiquitinating enzyme interaction landscape.

Sowa ME, Bennett EJ, Gygi SP, Harper JW

Deubiquitinating enzymes (Dubs) function to remove covalently attached ubiquitin from proteins, thereby controlling substrate activity and/or abundance. For most Dubs, their functions, targets, and regulation are poorly understood. To systematically investigate Dub function, we initiated a global proteomic analysis of Dubs and their associated protein complexes. This was accomplished through the development of a software platform called CompPASS, which uses ... [more]

Cell Jul. 23, 2009; 138(2);389-403 [Pubmed: 19615732]

Quantitative Score

1.57 [Confidence Score]

Throughput

High Throughput

Ontology Terms

hek-293 cell (BTO:0000007) [epithelial cell (BTO:0000414)]

Additional Notes

exogenous expression of bait

Curated By

BioGRID

Interaction Summary

USP46

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin thiolesterase activity [IDA]ubiquitin-specific protease activity [IDA]

YWHAH

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [IPI]glucocorticoid receptor binding [IPI]insulin-like growth factor receptor binding [ISS]ion channel binding [IPI]protein binding [IPI]protein domain specific binding [ISS]protein heterodimerization activity [IPI]sodium channel regulator activity [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS