An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Defining the human deubiquitinating enzyme interaction landscape.

Sowa ME, Bennett EJ, Gygi SP, Harper JW

Deubiquitinating enzymes (Dubs) function to remove covalently attached ubiquitin from proteins, thereby controlling substrate activity and/or abundance. For most Dubs, their functions, targets, and regulation are poorly understood. To systematically investigate Dub function, we initiated a global proteomic analysis of Dubs and their associated protein complexes. This was accomplished through the development of a software platform called CompPASS, which uses ... [more]

Cell Jul. 23, 2009; 138(2);389-403 [Pubmed: 19615732]

Quantitative Score

2.7 [Confidence Score]

Throughput

High Throughput

Ontology Terms

hek-293 cell (BTO:0000007) [epithelial cell (BTO:0000414)]

Additional Notes

exogenous expression of bait

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
USP45 RBMX	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Perez-Oliva AB (2015)	Low	-	BioGRID	1515246

Curated By

BioGRID

Interaction Summary

USP45

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [IBA]ubiquitin thiolesterase activity [IBA]ubiquitin-specific protease activity [IDA]

RBMX

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA, NAS]chromatin binding [IDA]core promoter binding [IDA]mRNA binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS