Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Defining the human deubiquitinating enzyme interaction landscape.

Sowa ME, Bennett EJ, Gygi SP, Harper JW

Deubiquitinating enzymes (Dubs) function to remove covalently attached ubiquitin from proteins, thereby controlling substrate activity and/or abundance. For most Dubs, their functions, targets, and regulation are poorly understood. To systematically investigate Dub function, we initiated a global proteomic analysis of Dubs and their associated protein complexes. This was accomplished through the development of a software platform called CompPASS, which uses ... [more]

Cell Jul. 23, 2009; 138(2);389-403 [Pubmed: 19615732]

Quantitative Score

  • 19.57 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007) [epithelial cell (BTO:0000414)]

Additional Notes

  • exogenous expression of bait

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AMOT NIN
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High205BioGRID
2978842

Curated By

  • BioGRID