BAIT
DBP5
RAT8, ATP-dependent RNA helicase DBP5, L000003292, YOR046C
Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
GO Process (2)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
ASF1
CIA1, nucleosome assembly factor ASF1, L000000126, YJL115W
Nucleosome assembly factor; involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition; relocalizes to the cytosol in response to hypoxia
GO Process (11)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- DNA replication-dependent nucleosome assembly [IDA]
- DNA replication-independent nucleosome assembly [IDA]
- chromatin silencing at silent mating-type cassette [IGI]
- chromatin silencing at telomere [IGI]
- histone H2B ubiquitination [IMP]
- histone acetylation [IMP]
- histone exchange [IMP]
- nucleosome disassembly [IMP]
- positive regulation of histone acetylation [IDA, IGI, IMP, IPI]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA]
- regulation of transcription from RNA polymerase II promoter in response to stress [IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components.
Coordination of the multiple steps of mRNA biogenesis helps to ensure proper regulation of gene expression. The Saccharomyces cerevisiae DEAD-box protein Rat8p/Dbp5p is an essential mRNA export factor that functions at the nuclear pore complex (NPC) where it is thought to remodel mRNA/protein complexes during mRNA export. Rat8p also functions in translation termination and has been implicated in functioning during ... [more]
Genetics Aug. 01, 2008; 179(4);1945-55 [Pubmed: 18689878]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- High Throughput: DBP5 is also known as RAT8. An SGA analysis was carried out using the temperature sensitive rat8-2 allele as the query.
Curated By
- BioGRID