RAT8, ATP-dependent RNA helicase DBP5, L000003292, YOR046C
Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
Saccharomyces cerevisiae (S288c)


Component of the INO80 chromatiin remodeling complex; target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.


Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components.

Scarcelli JJ, Viggiano S, Hodge CA, Heath CV, Amberg DC, Cole CN

Coordination of the multiple steps of mRNA biogenesis helps to ensure proper regulation of gene expression. The Saccharomyces cerevisiae DEAD-box protein Rat8p/Dbp5p is an essential mRNA export factor that functions at the nuclear pore complex (NPC) where it is thought to remodel mRNA/protein complexes during mRNA export. Rat8p also functions in translation termination and has been implicated in functioning during ... [more]

Genetics Aug. 01, 2008; 179(4);1945-55 [Pubmed: 18689878]


  • High Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • High Throughput: DBP5 is also known as RAT8. An SGA analysis was carried out using the temperature sensitive rat8-2 allele as the query.

Curated By

  • BioGRID