STUB1
Gene Ontology Biological Process
- cellular response to misfolded protein [IDA]
- misfolded or incompletely synthesized protein catabolic process [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- positive regulation of chaperone-mediated protein complex assembly [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein maturation [TAS]
- protein polyubiquitination [IDA, IMP]
- regulation of glucocorticoid metabolic process [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
Gene Ontology Cellular Component
AHR
Gene Ontology Biological Process
- apoptotic process [TAS]
- blood vessel development [NAS]
- circadian regulation of gene expression [ISS]
- intracellular receptor signaling pathway [IDA]
- negative regulation of transcription, DNA-templated [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IBA]
- positive regulation of transcription, DNA-templated [ISS]
- regulation of B cell proliferation [IDA]
- regulation of gene expression [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA]
- response to toxic substance [IDA]
- response to xenobiotic stimulus [IDA]
- transcription from RNA polymerase II promoter [IDA]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function- DNA binding [IDA, TAS]
- E-box binding [ISS]
- Hsp90 protein binding [IDA]
- enhancer binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein dimerization activity [TAS]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA, TAS]
- E-box binding [ISS]
- Hsp90 protein binding [IDA]
- enhancer binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein dimerization activity [TAS]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Carboxyl terminus of hsc70-interacting protein (CHIP) can remodel mature aryl hydrocarbon receptor (AhR) complexes and mediate ubiquitination of both the AhR and the 90 kDa heat-shock protein (hsp90) in vitro.
The regulation of the aryl hydrocarbon receptor (AhR) protein levels has been an area of keen interest, given its important role in mediating the cellular adaptation and toxic response to several environmental pollutants. The carboxyl terminus of hsc70-interacting protein (CHIP) ubiquitin ligase was previously associated with the regulation of the aryl hydrocarbon receptor, although the mechanisms were not directly demonstrated. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AHR STUB1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID