BAIT

SARNP

CIP29, HCC1, HSPC316, THO1
SAP domain containing ribonucleoprotein
GO Process (1)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

FUS

ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS
FUS RNA binding protein
GO Process (3)
GO Function (4)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Intracellular characterization of DDX39, a novel growth-associated RNA helicase.

Sugiura T, Sakurai K, Nagano Y

DDX39 belongs to the DEAD box RNA helicase family and is overexpressed in human lung squamous cell carcinoma. In this study, in order to seek the biological relevance of DDX39, we conducted its intracellular characterization. When expressed in 293 cells, DDX39 undergoes heavy ubiquitylation and the stability of DDX39 is regulated via a ubiqutin-proteasome pathway. DDX39 tethers ALY, an essential ... [more]

Exp. Cell Res. Feb. 15, 2007; 313(4);782-90 [Pubmed: 17196963]

Throughput

  • Low Throughput

Curated By

  • BioGRID