BAIT

PMS1

HNPCC3, PMSL1, hPMS1
PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
GO Process (2)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Characterization of the interactome of the human MutL homologues MLH1, PMS1, and PMS2.

Cannavo E, Gerrits B, Marra G, Schlapbach R, Jiricny J

Postreplicative mismatch repair (MMR) involves the concerted action of at least 20 polypeptides. Although the minimal human MMR system has recently been reconstituted in vitro, genetic evidence from different eukaryotic organisms suggests that some steps of the MMR process may be carried out by more than one protein. Moreover, MMR proteins are involved also in other pathways of DNA metabolism, ... [more]

J. Biol. Chem. Feb. 02, 2007; 282(5);2976-86 [Pubmed: 17148452]

Throughput

  • Low Throughput

Curated By

  • BioGRID