An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Copper-dependent interaction of dynactin subunit p62 with the N terminus of ATP7B but not ATP7A.

Lim CM, Cater MA, Mercer JF, La Fontaine S

The P-type ATPase affected in Wilson disease, ATP7B, is a key liver protein required to regulate and maintain copper homeostasis. When hepatocytes are exposed to elevated copper levels, ATP7B traffics from the trans-Golgi network toward the biliary canalicular membrane to excrete excess copper into bile. The N-terminal region of ATP7B contains six metal-binding sites (MBS), each with the copper-binding motif ... [more]

J. Biol. Chem. May. 19, 2006; 281(20);14006-14 [Pubmed: 16554302]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ATP7B DCTN4	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Lim CM (2006)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ATP7B

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]copper ion binding [IDA]copper-exporting ATPase activity [NAS, TAS]protein binding [IPI]

Gene Ontology Cellular Component

DCTN4