BAIT

HTA1

H2A1, SPT11, histone H2A, L000000827, YDR225W
Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

HIS5

histidinol-phosphate transaminase, L000000782, YIL116W
Histidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Alterations in DNA replication and histone levels promote histone gene amplification in Saccharomyces cerevisiae.

Libuda DE, Winston F

Gene amplification, a process that increases the copy number of a gene or a genomic region to two or more, is utilized by many organisms in response to environmental stress or decreased levels of a gene product. Our previous studies in Saccharomyces cerevisiae identified the amplification of a histone H2A-H2B gene pair, HTA2-HTB2, in response to the deletion of the ... [more]

Genetics Apr. 01, 2010; 184(4);985-97 [Pubmed: 20139344]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: viability (APO:0000111)

Additional Notes

  • A synthetic genetic array (SGA) analysis was performed to identify deletions that either increase or decrease/eliminate the viability of an hta1 htb1 double mutant. Since HTA2 -HTB2 gene amplification is required for survival in an hta1 htb1 double mutant background, the level of survival of the triple mutant served as an indicator of the frequency of HTA2-HTB2 amplification.

Curated By

  • BioGRID