BAIT

HTB1

SPT12, histone H2B, L000000829, YDR224C
Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

CSM2

YIL132C
Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Shu1, Shu2, and promotes error-free DNA repair,; Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Alterations in DNA replication and histone levels promote histone gene amplification in Saccharomyces cerevisiae.

Libuda DE, Winston F

Gene amplification, a process that increases the copy number of a gene or a genomic region to two or more, is utilized by many organisms in response to environmental stress or decreased levels of a gene product. Our previous studies in Saccharomyces cerevisiae identified the amplification of a histone H2A-H2B gene pair, HTA2-HTB2, in response to the deletion of the ... [more]

Genetics Apr. 01, 2010; 184(4);985-97 [Pubmed: 20139344]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: viability (APO:0000111)

Additional Notes

  • A synthetic genetic array (SGA) analysis was performed to identify deletions that either increase or decrease/eliminate the viability of an hta1 htb1 double mutant. Since HTA2 -HTB2 gene amplification is required for survival in an hta1 htb1 double mutant background, the level of survival of the triple mutant served as an indicator of the frequency of HTA2-HTB2 amplification.

Curated By

  • BioGRID