BAIT

HTB1

SPT12, histone H2B, L000000829, YDR224C
Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

DPB3

L000000520, YBR278W
Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Alterations in DNA replication and histone levels promote histone gene amplification in Saccharomyces cerevisiae.

Libuda DE, Winston F

Gene amplification, a process that increases the copy number of a gene or a genomic region to two or more, is utilized by many organisms in response to environmental stress or decreased levels of a gene product. Our previous studies in Saccharomyces cerevisiae identified the amplification of a histone H2A-H2B gene pair, HTA2-HTB2, in response to the deletion of the ... [more]

Genetics Apr. 01, 2010; 184(4);985-97 [Pubmed: 20139344]

Throughput

  • Low Throughput

Ontology Terms

  • viability (APO:0000111)

Additional Notes

  • Low Throughput: Tetrad analysis confirmed that the deletion of either DPB3, DPB4, RRM3 or CLB5 increased the viability of an hta1 htb1 double mutant.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HTB1 DPB3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
428107

Curated By

  • BioGRID