BAIT

PSTPIP1

CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPAS, PSTPIP
proline-serine-threonine phosphatase interacting protein 1
GO Process (3)
GO Function (1)
GO Component (2)
Homo sapiens
PREY

DNM2

CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5
dynamin 2
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of interaction partners for individual SH3 domains of Fas ligand associated members of the PCH protein family in T lymphocytes.

Linkermann A, Gelhaus C, Lettau M, Qian J, Kabelitz D, Janssen O

Pombe Cdc15 homology (PCH) family proteins are regarded as key elements for linking membrane-associated processes to cytoskeletal elements and thus play a major role in exo- and endocytosis and organelle trafficking. We previously reported that, via their SH3 domains, several members of the PCH proteins interact with the proline-rich region of Fas ligand (FasL, CD95L), a key death factor in ... [more]

Biochim. Biophys. Acta Feb. 01, 2009; 1794(2);168-76 [Pubmed: 19041431]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSTPIP1 DNM2
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID