BAIT
TRP53
Tp53, bbl, bfy, bhy, p44, p53, RP23-56I20.1
transformation related protein 53
GO Process (100)
GO Function (32)
GO Component (17)
Gene Ontology Biological Process
- B cell lineage commitment [IMP]
- DNA damage response, signal transduction by p53 class mediator [IDA, IGI, ISO]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IGI]
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [IBA, ISO]
- DNA strand renaturation [ISO]
- ER overload response [ISO]
- T cell differentiation in thymus [IGI]
- T cell lineage commitment [IMP]
- T cell proliferation involved in immune response [IGI]
- apoptotic process [IDA, IGI]
- cell aging [IGI, ISO]
- cell cycle arrest [ISO]
- cellular protein localization [ISO]
- cellular response to DNA damage stimulus [IDA, IMP, ISO]
- cellular response to UV [IGI]
- cellular response to glucose starvation [ISO]
- cellular response to ionizing radiation [IGI, ISO]
- central nervous system development [IGI]
- cerebellum development [IDA]
- chromatin assembly [ISO]
- chromosome organization [IGI]
- determination of adult lifespan [IMP]
- double-strand break repair [IMP]
- embryo development ending in birth or egg hatching [IGI]
- embryonic organ development [IGI]
- gastrulation [IGI]
- in utero embryonic development [IGI]
- intrinsic apoptotic signaling pathway by p53 class mediator [IDA, IGI, ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IGI, IMP, ISO]
- mitochondrial DNA repair [IMP]
- mitotic G1 DNA damage checkpoint [IMP, ISO]
- mitotic cell cycle arrest [IDA]
- multicellular organism growth [IGI]
- multicellular organismal development [ISO]
- necroptotic process [IGI]
- negative regulation of DNA biosynthetic process [ISO]
- negative regulation of DNA replication [IDA, IMP]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IGI, IMP]
- negative regulation of fibroblast proliferation [IMP, ISO]
- negative regulation of macromitophagy [IMP]
- negative regulation of mitotic cell cycle [IGI]
- negative regulation of neuroblast proliferation [IGI]
- negative regulation of proteolysis [IMP]
- negative regulation of reactive oxygen species metabolic process [IMP]
- negative regulation of smooth muscle cell proliferation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- negative regulation of transcription, DNA-templated [IMP, ISO]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP]
- neuron apoptotic process [IGI, IMP]
- nucleotide-excision repair [ISO]
- oligodendrocyte apoptotic process [ISO]
- oxidative stress-induced premature senescence [ISO]
- positive regulation of apoptotic process [IGI, IMP, ISO]
- positive regulation of cardiac muscle cell apoptotic process [IMP]
- positive regulation of cell aging [IMP]
- positive regulation of cell cycle [ISO]
- positive regulation of cell cycle arrest [ISO]
- positive regulation of histone deacetylation [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [ISO]
- positive regulation of leukocyte migration [ISO]
- positive regulation of mitochondrial membrane permeability [IGI]
- positive regulation of neuron apoptotic process [IDA, IGI, IMP]
- positive regulation of peptidyl-tyrosine phosphorylation [IMP]
- positive regulation of protein oligomerization [ISO]
- positive regulation of reactive oxygen species metabolic process [ISO]
- positive regulation of release of cytochrome c from mitochondria [ISO]
- positive regulation of thymocyte apoptotic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IBA, IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA, IGI, ISO]
- protein complex assembly [ISO]
- protein import into nucleus, translocation [IDA]
- protein localization [ISO]
- rRNA transcription [IGI]
- regulation of apoptotic process [ISO]
- regulation of catalytic activity [IDA]
- regulation of cell cycle [IGI, IMP]
- regulation of cell proliferation [IGI, IMP]
- regulation of fibroblast apoptotic process [IGI]
- regulation of intracellular pH [ISO]
- regulation of intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- regulation of mitochondrial membrane permeability involved in apoptotic process [IGI]
- regulation of neuron apoptotic process [IGI]
- regulation of thymocyte apoptotic process [IGI]
- regulation of tissue remodeling [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA, ISO]
- release of cytochrome c from mitochondria [IDA, IGI]
- replicative senescence [ISO]
- response to UV [IDA, IMP]
- response to X-ray [IDA]
- response to drug [IDA]
- response to gamma radiation [IDA, IMP, ISO]
- response to ischemia [IMP]
- response to oxidative stress [IGI, IMP]
- response to salt stress [IGI]
- somitogenesis [IGI]
- transcription from RNA polymerase II promoter [IGI]
- transforming growth factor beta receptor signaling pathway [IGI]
Gene Ontology Molecular Function- ATP binding [ISO]
- DNA binding [IDA, ISO]
- MDM2/MDM4 family protein binding [IPI, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IGI]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- chaperone binding [ISO]
- chromatin binding [IDA, ISO]
- copper ion binding [ISO]
- damaged DNA binding [IBA]
- double-stranded DNA binding [IBA]
- enzyme binding [ISO]
- histone acetyltransferase binding [ISO]
- histone deacetylase regulator activity [IDA]
- identical protein binding [ISO]
- p53 binding [IBA]
- protease binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [ISO]
- protein phosphatase binding [ISO]
- receptor tyrosine kinase binding [ISO]
- sequence-specific DNA binding [IBA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription cofactor binding [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ATP binding [ISO]
- DNA binding [IDA, ISO]
- MDM2/MDM4 family protein binding [IPI, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IGI]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- chaperone binding [ISO]
- chromatin binding [IDA, ISO]
- copper ion binding [ISO]
- damaged DNA binding [IBA]
- double-stranded DNA binding [IBA]
- enzyme binding [ISO]
- histone acetyltransferase binding [ISO]
- histone deacetylase regulator activity [IDA]
- identical protein binding [ISO]
- p53 binding [IBA]
- protease binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [ISO]
- protein phosphatase binding [ISO]
- receptor tyrosine kinase binding [ISO]
- sequence-specific DNA binding [IBA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription cofactor binding [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- PML body [ISO]
- chromatin [IBA, ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- intracellular [IGI]
- mitochondrial matrix [IDA]
- mitochondrion [IBA, IDA, IGI, ISO]
- nuclear body [ISO]
- nuclear chromatin [ISO]
- nuclear matrix [ISO]
- nucleolus [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- protein complex [ISO]
- replication fork [IDA]
- transcription factor TFIID complex [ISO]
- transcription factor complex [IBA, ISO]
Mus musculus
PREY
UBE2N
1500026J17Rik, AL022654, BB101821, UBC13
ubiquitin-conjugating enzyme E2N
GO Process (14)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- DNA double-strand break processing [ISO]
- T cell receptor signaling pathway [ISO, ISS]
- double-strand break repair via homologous recombination [ISO]
- histone ubiquitination [ISO]
- positive regulation of DNA repair [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO, ISS]
- positive regulation of histone modification [ISO]
- positive regulation of ubiquitin-protein transferase activity [ISO]
- postreplication repair [IBA, ISO]
- protein K63-linked ubiquitination [IBA, ISO]
- protein ubiquitination [ISO]
- regulation of histone ubiquitination [ISO]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Regulation of p53 localization and activity by Ubc13.
The abundance and activity of p53 are regulated largely by ubiquitin ligases. Here we demonstrate a previously undisclosed regulation of p53 localization and activity by Ubc13, an E2 ubiquitin-conjugating enzyme. While increasing p53 stability, Ubc13 decreases p53 transcriptional activity and increases its localization to the cytoplasm, changes that require its ubiquitin-conjugating activity. Ubc13 elicits K63-dependent ubiquitination of p53, which attenuates ... [more]
Mol. Cell. Biol. Dec. 01, 2006; 26(23);8901-13 [Pubmed: 17000756]
Throughput
- Low Throughput
Curated By
- BioGRID