BAIT

GGA1

RP1-37E16.3
golgi-associated, gamma adaptin ear containing, ARF binding protein 1
Alzheimer's Disease Project
UPS Project
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

VPS18

PEP3
vacuolar protein sorting 18 homolog (S. cerevisiae)
Autophagy Project
UPS Project
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability.

Yogosawa S, Kawasaki M, Wakatsuki S, Kominami E, Shiba Y, Nakayama K, Kohsaka S, Akazawa C

GGAs (Golgi-localizing, gamma-adaptin ear domain homology, ADP-ribosylation factor (ARF)-binding proteins), constitute a family of monomeric adaptor proteins and are associated with protein trafficking from the trans-Golgi network to endosomes. Here, we show that GGA3 is monoubiquitylated by a RING-H2 type-ubiquitin ligase hVPS18 (human homologue of vacuolar protein sorting 18). By in vitro ubiquitylation assays, we have identified lysine 258 in ... [more]

Biochem. Biophys. Res. Commun. Nov. 10, 2006; 350(1);82-90 [Pubmed: 16996030]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GGA1 VPS18
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID