PREY

TIRAP

BACTS1, Mal, MyD88-2, wyatt
toll-interleukin 1 receptor (TIR) domain containing adaptor protein
GO Process (35)
GO Function (8)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Triad3A regulates ubiquitination and proteasomal degradation of RIP1 following disruption of Hsp90 binding.

Fearns C, Pan Q, Mathison JC, Chuang TH

Toll-like receptors (TLRs) play a crucial role in innate immunity by recognizing microbial pathogens. Triad3A is an E3 ubiquitin-protein ligase that interacts with the Toll/interleukin-1 receptor domain of TLRs and promotes their proteolytic degradation. In the present study, we further investigated its activity on signaling molecules downstream of TLRs and tumor necrosis factor (TNF) receptor 1. Triad3A promoted down-regulation of ... [more]

J. Biol. Chem. Nov. 10, 2006; 281(45);34592-600 [Pubmed: 16968706]

Throughput

  • Low Throughput

Curated By

  • BioGRID