RAD3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
POL32
Gene Ontology Biological Process
- DNA amplification [IMP]
- DNA replication, removal of RNA primer [IDA]
- RNA-dependent DNA replication [IDA]
- base-excision repair [TAS]
- double-strand break repair via break-induced replication [IMP]
- lagging strand elongation [TAS]
- leading strand elongation [TAS]
- mismatch repair [NAS]
- nucleotide-excision repair [TAS]
- postreplication repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms.
Homologous recombination is a major double-strand break (DSB) repair mechanism that acts during the S and G2 phases. In contrast, nucleotide excision repair (NER) is a major pathway for the repair of DNA bulky adducts that is unrelated to replication. We show that replication can be strongly disturbed in a specific type of rad3/XPD NER mutant of TFIIH, causing replication ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- Deletion of POL32 causes synthetic lethality in a RAD3/RAD51 double mutant background
- genetic complex
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
POL32 RAD3 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 4.9223 | BioGRID | 225142 |
Curated By
- BioGRID