CTCF
Gene Ontology Biological Process
- DNA methylation [IDA, IMP]
- dosage compensation by inactivation of X chromosome [TAS]
- maintenance of DNA methylation [IMP]
- negative regulation of gene expression [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- nucleosome positioning [ISO]
- positive regulation of gene expression [IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of gene expression by genetic imprinting [IDA, IMP]
- regulation of gene expression, epigenetic [ISO]
- regulation of histone acetylation [IMP]
- regulation of histone methylation [IMP]
- regulation of molecular function, epigenetic [ISO]
- regulation of transcription, DNA-templated [IDA]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin binding [IDA]
- chromatin insulator sequence binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription regulatory region DNA binding [ISO]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin binding [IDA]
- chromatin insulator sequence binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription regulatory region DNA binding [ISO]
Gene Ontology Cellular Component
ATRX
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- DNA damage response, signal transduction by p53 class mediator [IMP]
- DNA replication-independent nucleosome assembly [ISO]
- Sertoli cell development [IMP]
- cellular response to hydroxyurea [IMP]
- chromatin remodeling [ISO]
- forebrain development [IMP]
- negative regulation of telomeric RNA transcription from RNA pol II promoter [IMP]
- nucleosome assembly [ISO]
- positive regulation of nuclear cell cycle DNA replication [IMP]
- positive regulation of telomere maintenance [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IGI, ISO]
- replication fork processing [IMP]
- seminiferous tubule development [IMP]
- spermatogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
ATRX partners with cohesin and MeCP2 and contributes to developmental silencing of imprinted genes in the brain.
Human developmental disorders caused by chromatin dysfunction often display overlapping clinical manifestations, such as cognitive deficits, but the underlying molecular links are poorly defined. Here, we show that ATRX, MeCP2, and cohesin, chromatin regulators implicated in ATR-X, RTT, and CdLS syndromes, respectively, interact in the brain and colocalize at the H19 imprinting control region (ICR) with preferential binding on the ... [more]
Throughput
- Low Throughput
Additional Notes
- chromatin immunoprecipitation
Curated By
- BioGRID