CDC6
Gene Ontology Biological Process
- DNA replication [TAS]
- DNA replication checkpoint [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- mitotic cell cycle [TAS]
- negative regulation of DNA replication [TAS]
- negative regulation of cell proliferation [TAS]
- positive regulation of chromosome segregation [IDA]
- positive regulation of cytokinesis [IMP]
- regulation of cyclin-dependent protein serine/threonine kinase activity [TAS]
- regulation of mitotic metaphase/anaphase transition [IMP]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [TAS]
- traversing start control point of mitotic cell cycle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ATR
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells.
CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by phosphorylation by Cdk. CDC6 silencing by siRNAs affected ATR-dependent inhibition ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID