RPN11
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ABF1
Gene Ontology Biological Process
- DNA-dependent DNA replication [IDA]
- chromatin remodeling [IDA, IGI]
- chromatin silencing at silent mating-type cassette [IMP]
- global genome nucleotide-excision repair [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding, bending [IDA]
- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding, bending [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Characterization of cell cycle specific protein interaction networks of the yeast 26S proteasome complex by the QTAX strategy.
Ubiquitin-proteasome dependent protein degradation plays a fundamental role in the regulation of the eukaryotic cell cycle. Cell cycle transitions between different phases are tightly regulated to prevent uncontrolled cell proliferation, which is characteristic of cancer cells. To understand cell cycle phase specific regulation of the 26S proteasome and reveal the molecular mechanisms underlying the ubiquitin-proteasome degradation pathway during cell cycle ... [more]
Throughput
- High Throughput
Additional Notes
- Proteasome interacting proteins (PIPs) were identified using QTAX (quantitative analysis of tandem affinity purified in vivo cross-linked (X) protein complexes). TAP-tagged Rpn11 and in vivo formaldehyde cross-linking were used in the experiment.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ABF1 RPN11 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3892 | BioGRID | 1941220 |
Curated By
- BioGRID