BAIT

CSC1

YLR241W, YLR241W
Calcium permeable gated cation channel; may be involved in detoxification; similar to Arabidopsis CSC1
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)
PREY

NEO1

putative aminophospholipid-translocating P4-type ATPase NEO1, L000004112, YIL048W
Putative aminophospholipid translocase (flippase); involved in endocytosis, vacuolar biogenesis and Golgi to ER vesicle-mediated transport; localizes to endosomes and the Golgi apparatus
Saccharomyces cerevisiae (S288c)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Large-scale identification of yeast integral membrane protein interactions.

Miller JP, Lo RS, Ben-Hur A, Desmarais C, Stagljar I, Noble WS, Fields S

We carried out a large-scale screen to identify interactions between integral membrane proteins of Saccharomyces cerevisiae by using a modified split-ubiquitin technique. Among 705 proteins annotated as integral membrane, we identified 1,985 putative interactions involving 536 proteins. To ascribe confidence levels to the interactions, we used a support vector machine algorithm to classify interactions based on the assay results and ... [more]

Proc. Natl. Acad. Sci. U.S.A. Aug. 23, 2005; 102(34);12123-8 [Pubmed: 16093310]

Throughput

  • High Throughput

Additional Notes

  • A large-scale split-ubiquitin screen was performed to identify interactions between integral membrane proteins.

Curated By

  • BioGRID