BAIT
DOA4
DOS1, MUT4, NPI2, SSV7, UBP4, L000000514, YDR069C
Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication
GO Process (7)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- endocytosis [IMP]
- free ubiquitin chain depolymerization [IDA]
- intralumenal vesicle formation [IGI]
- regulation of DNA replication [IMP]
- ubiquitin homeostasis [IMP]
- ubiquitin-dependent protein catabolic process [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
POL1
CDC17, CRT5, HPR3, DNA-directed DNA polymerase alpha catalytic subunit POL1, L000000257, YNL102W
Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
GO Process (11)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- DNA replication [IMP]
- DNA replication checkpoint [IBA]
- DNA replication initiation [IC]
- DNA replication, synthesis of RNA primer [IBA]
- DNA strand elongation involved in DNA replication [IBA]
- DNA synthesis involved in DNA repair [IMP]
- RNA-dependent DNA replication [IDA]
- double-strand break repair [IMP]
- gene conversion at mating-type locus, DNA repair synthesis [IBA]
- lagging strand elongation [IC]
- premeiotic DNA replication [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism.
We have developed a genetic screen of the yeast Saccharomyces cerevisiae to identify genes that act to coordinate DNA replication so that each part of the genome is copied exactly once per cell cycle. A mutant was recovered in this screen that accumulates aberrantly high DNA contents but does not complete a second round of synthesis. The mutation principally responsible ... [more]
Mol. Cell. Biol. Apr. 01, 1996; 16(4);1356-66 [Pubmed: 8657109]
Throughput
- Low Throughput
Ontology Terms
- phenotype: heat sensitivity (APO:0000147)
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID