An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The PcG protein hPc2 interacts with the N-terminus of histone demethylase JARID1B and acts as a transcriptional co-repressor.

Zhou W, Chen H, Zhang L

JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. We demonstrated that ... [more]

BMB Rep Mar. 31, 2009; 42(3);154-9 [Pubmed: 19336002]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KDM5B CDX2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Faber K (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

KDM5B

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone demethylase activity (H3-dimethyl-K4 specific) [IDA]histone demethylase activity (H3-trimethyl-K4 specific) [IDA]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]transcription corepressor activity [IDA]

Gene Ontology Cellular Component

CDX2