BAIT

ECM1

L000003052, YAL059W
Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

YPR108W-A

Putative protein of unknown function; identified by fungal homology and RT-PCR
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export.

Yao Y, Demoinet E, Saveanu C, Lenormand P, Jacquier A, Fromont-Racine M

In eukaryotes, ribosome biogenesis is a highly conserved process that starts in the nucleus and ends in the cytoplasm. In actively growing yeast cells, it is estimated that each nuclear pore complex (NPC) contributes to the export of about 25 pre-ribosomal particles per minute. Such an extremely active process requires several redundant export receptors for the pre-60S particles. Here, we ... [more]

RNA May. 01, 2010; 16(5);1007-17 [Pubmed: 20348449]

Throughput

  • Low Throughput

Curated By

  • BioGRID