PREY

NEDD4

Nedd4a
neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
GO Process (35)
GO Function (12)
GO Component (13)

Gene Ontology Biological Process

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RNA interference screen reveals an essential role of Nedd4-2 in dopamine transporter ubiquitination and endocytosis.

Sorkina T, Miranda M, Dionne KR, Hoover BR, Zahniser NR, Sorkin A

The function of the dopamine transporter (DAT) to terminate dopamine neurotransmission is regulated by endocytic trafficking of DAT. To elucidate the mechanisms of DAT endocytosis, we generated a fully functional mutant of the human DAT in which a hemagglutinin epitope (HA) was incorporated into the second extracellular loop. The endocytosis assay, based on the uptake of an HA antibody, was ... [more]

J. Neurosci. Aug. 02, 2006; 26(31);8195-205 [Pubmed: 16885233]

Throughput

  • Low Throughput

Curated By

  • BioGRID