SLC6A3
Gene Ontology Biological Process
- adenohypophysis development [ISO]
- aging [IEP]
- cation transmembrane transport [IDA, ISO]
- dopamine biosynthetic process [ISO]
- dopamine catabolic process [ISO]
- dopamine transport [IDA, ISO]
- lactation [ISO]
- locomotory behavior [ISO]
- monoamine transport [ISO]
- positive regulation of multicellular organism growth [ISO]
- prepulse inhibition [ISO]
- regulation of dopamine metabolic process [ISO]
- response to cAMP [IDA]
- response to cocaine [ISO]
- response to drug [IEP]
- response to ethanol [IDA]
- response to iron ion [IEP]
- response to nicotine [IEP]
- response to organic cyclic compound [IEP]
- sensory perception of smell [ISO]
Gene Ontology Molecular Function- dopamine binding [ISO]
- dopamine transmembrane transporter activity [IDA, ISO]
- dopamine:sodium symporter activity [IDA]
- drug binding [IDA]
- monoamine transmembrane transporter activity [ISO]
- protease binding [IPI]
- protein N-terminus binding [ISO]
- protein complex binding [IDA]
- protein phosphatase 2A binding [IPI]
- receptor binding [IPI]
- dopamine binding [ISO]
- dopamine transmembrane transporter activity [IDA, ISO]
- dopamine:sodium symporter activity [IDA]
- drug binding [IDA]
- monoamine transmembrane transporter activity [ISO]
- protease binding [IPI]
- protein N-terminus binding [ISO]
- protein complex binding [IDA]
- protein phosphatase 2A binding [IPI]
- receptor binding [IPI]
Gene Ontology Cellular Component
EPN1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
RNA interference screen reveals an essential role of Nedd4-2 in dopamine transporter ubiquitination and endocytosis.
The function of the dopamine transporter (DAT) to terminate dopamine neurotransmission is regulated by endocytic trafficking of DAT. To elucidate the mechanisms of DAT endocytosis, we generated a fully functional mutant of the human DAT in which a hemagglutinin epitope (HA) was incorporated into the second extracellular loop. The endocytosis assay, based on the uptake of an HA antibody, was ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID