A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Functional modules in the Arabidopsis core cell cycle binary protein-protein interaction network.

Boruc J, Van den Daele H, Hollunder J, Rombauts S, Mylle E, Hilson P, Inze D, De Veylder L, Russinova E

As in other eukaryotes, cell division in plants is highly conserved and regulated by cyclin-dependent kinases (CDKs) that are themselves predominantly regulated at the posttranscriptional level by their association with proteins such as cyclins. Although over the last years the knowledge of the plant cell cycle has considerably increased, little is known on the assembly and regulation of the different ... [more]

Plant Cell Apr. 01, 2010; 22(4);1264-80 [Pubmed: 20407024]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KRP2 CDC2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Van Leene J (2010)	High	-	BioGRID	-
KRP2 CDC2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Verkest A (2005)	Low	-	BioGRID	-
KRP2 CDC2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Verkest A (2005)	Low	-	BioGRID	-
CDC2 KRP2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Boruc J (2010)	High	-	BioGRID	-
CDC2 KRP2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Lui H (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

cyclin-dependent protein serine/threonine kinase activity [ISS]kinase activity [IMP, ISS]protein binding [IPI]protein kinase activity [IDA]

Gene Ontology Cellular Component

KRP2