A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Functional modules in the Arabidopsis core cell cycle binary protein-protein interaction network.

Boruc J, Van den Daele H, Hollunder J, Rombauts S, Mylle E, Hilson P, Inze D, De Veylder L, Russinova E

As in other eukaryotes, cell division in plants is highly conserved and regulated by cyclin-dependent kinases (CDKs) that are themselves predominantly regulated at the posttranscriptional level by their association with proteins such as cyclins. Although over the last years the knowledge of the plant cell cycle has considerably increased, little is known on the assembly and regulation of the different ... [more]

Plant Cell Apr. 01, 2010; 22(4);1264-80 [Pubmed: 20407024]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ICK1 CKS2	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Boruc J (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CKS2

Gene Ontology Molecular Function

cyclin-dependent protein serine/threonine kinase activity [ISS]protein binding [IPI]

Gene Ontology Cellular Component

ICK1

Gene Ontology Biological Process