Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HDAC1 nuclear export induced by pathological conditions is essential for the onset of axonal damage.

Kim JY, Shen S, Dietz K, He Y, Howell O, Reynolds R, Casaccia P

Histone deacetylase 1 (HDAC1) is a nuclear enzyme involved in transcriptional repression. We detected cytosolic HDAC1 in damaged axons in brains of humans with multiple sclerosis and of mice with cuprizone-induced demyelination, in ex vivo models of demyelination and in cultured neurons exposed to glutamate and tumor necrosis factor-alpha. Nuclear export of HDAC1 was mediated by the interaction with the ... [more]

Nat. Neurosci. Feb. 01, 2010; 13(2);180-9 [Pubmed: 20037577]

Throughput

  • Low Throughput

Curated By

  • BioGRID