BAIT

DST1

PPR2, SII, S-II, TFIIS, P37, L000001476, L000000530, YGL043W
General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
Saccharomyces cerevisiae (S288c)
PREY

SNG1

L000002747, YGR197C
Protein involved in resistance to nitrosoguanidine and 6-azauracil; expression is regulated by transcription factors involved in multidrug resistance; SNG1 has a paralog, YJR015W, that arose from the whole genome duplication
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Overexpression of SNG1 causes 6-azauracil resistance in Saccharomyces cerevisiae.

Garcia-Lopez MC, Miron-Garcia MC, Garrido-Godino AI, Mingorance C, Navarro F

The mechanism of action of 6AU, a growth inhibitor for many microorganisms causing depletion of intracellular nucleotide pools of GTP and UTP, is not well understood. To gain insight into the mechanisms leading to 6AU resistance, and in an attempt to uncover novel genes required for this resistance, we undertook a high-copy-number suppressor screening to identify genes whose overexpression could ... [more]

Curr. Genet. Jun. 01, 2010; 56(3);251-63 [Pubmed: 20424846]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • overexpression decreases sensitivity to 6AU

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SNG1 DST1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3196BioGRID
2122705

Curated By

  • BioGRID