BAIT

PSMA6

IOTA, PROS27, p27K
proteasome (prosome, macropain) subunit, alpha type, 6
GO Process (24)
GO Function (5)
GO Component (13)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Modification of Cul1 regulates its association with proteasomal subunits.

Bloom J, Peschiaroli A, Demartino G, Pagano M

ABSTRACT : BACKGROUND : Ubiquitylation targets proteins for degradation by the 26S proteasome. Some yeast and plant ubiquitin ligases, including the highly conserved SCF (Skp1/Cul1/F-box protein) complex, have been shown to associate with proteasomes. We sought to characterize interactions between SCF complexes and proteasomes in mammalian cells. RESULTS : We found that the binding of SCF complexes to proteasomes is ... [more]

Unknown Jun. 09, 2006; 1(0);5 [Pubmed: 16759355]

Throughput

  • Low Throughput

Curated By

  • BioGRID